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Kristen J. Skogerboe, a Professor of Chemistry at Seattle University, received her Ph.D. in Analytical Chemistry in 1986 from Iowa State University. She completed post-doctoral studies in Clinical Chemistry and Clinical Molecular Genetics at the University of Washington. For six years she served as a laboratory director of Molecular Biology at Dynacare Laboratory of Pathology, and joined the Seattle University faculty in 1995. She is board certified in Clinical Chemistry (DABCC) and Molecular Medical Genetics (DABMG). She was Chair of the Department from 2003 - 2007. She is interested in developing sensitive methods for the analysis of biologic molecules, and in promoting active learning in the chemistry curriculum.

BIOGRAPHICAL SKETCH

Curriculum Vitae

Skogerboe, Kristen Johanna Department of Chemistry Seattle University 900 Broadway Seattle, WA 98122		Professor of Chemistry Diplomate of American Board Clinical Chemistry Diplomate of American Board Medical Genetics skogerbo@seattleu.edu (206) 296-6360
EDUCATION/TRAINING
INSTITUTION AND LOCATION	DEGREE		YEAR(s)	FIELD OF STUDY
Colorado State University, Fort Collins CO	B.S.		1978-1982	Chemistry
Iowa State University, Ames IA	Ph.D.		1982-1986	Chemistry
University of Washington, Seattle WA			1986-1988	Clinical Chemistry
University of Washington, Seattle WA			1988-1990	Molecular Genetics

Professional Positions

Professor, Department of Chemistry 2007-Present

Associate Professor 2001– 2007

Departmental Chairperson 2003– Present

Assistant Professor 1999– 2001

Seattle University, Seattle WA

Research in surfactant proteins and interfacial chemistry; Teaching General

Chemistry, Analytical Chemistry, Forensic Science and Active Learning

Visiting Associate Professor, Department of Chemistry 2002 - 2003

University of Washington (Sabbatical Research Appointment) 1999, 2002-2006

Capillary electrophoresis studies of protein expression in single (Summers)

neurons exposed to cannabinoids and Teaching Quantitative Analysis

Visiting Research Scientist 1998

Childrens Hospital and Medical Center, Seattle WA (Summer)

Clinical studies in pediatric oncology

Associate Director, Scientific and Technical 1991 - 1995

Affairs, Molecular Biology Department (1995-2003*)

Lab Corp, Dynacare, Lab of Pathology and Swedish Medical Center (*consulting)

Clinical Molecular Genetics, Virology, Oncology and Paternity Testing

Senior Clinical Chemistry Fellow and Molecular Genetics Fellow 1986 - 1990

University of Washington, Seattle, WA

Fellowships in Clinical Chemistry and Molecular Genetics

Research Assistant 1982-1986

Dept of Energy Ames National Lab, Iowa State University, Ames IA

Graduate Research in Analytical Chemistry

Research Associate 1980-1981

The Procter and Gamble Company

Cincinatti OH

Personal Products Research, Detergents and Surfactants

SELECTED MEMBERSHIPS/ACTIVITIES/CONFERENCES

External to Seattle University

· Member: American Chemical Society (ACS) 1982-present

· Member: American Association of Clinical Chemistry (AACC) 1986-present

· National Abstracts Chair: AACC (2006)

· Annual Meeting Organizing Committee: AACC (2005-2006); Chair of the PNW Region (1993 – 1994); Secretary of National Molecular Pathology Division (1995-1996)

· Panel Reviewer: NSF Division of Undergraduate Education CCLI, Washington DC (Jan 2005)

· Sunday School Teacher: Sand Point United Methodist Church (2003-present)

· Appointed Member: Washington State Dept. of Health Genetics Advisory Council (2003 – Present)

· Session Presenter: Johns Hopkins University Center for Talented Youth Odyssey Series (April 2006)

· Huckaby Fellowship Panelist/Reviewer: Graduate College, Univ.of Washington (2001-2004, 2006)

· Conference Attendee: American Association of Clinical Chemistry National Meetings: Orlando, Florida and Chicago, Illinois, earning over 50 hours of continuing education (2005, 2006)

· Institutional participant: International conference on the Scholarship of Teaching and Learning, Vancouver British Columbia, (Nov 2005)

· Workshop Participant: Process Oriented Guided Inquiry of Learning (POGIL) workshops supported by the National Science Foundation, Seattle WA, (2004, 2006)

· Invited Conference Participant: “Research Corporation: Models in Academic Leadership” Tucson Arizona (July 2004)

· Conference Presentation and Session Presider: Biennial Conference of Chemical Education,” Using the World of the Working Chemist to Reform the Undergraduate Analytical Laboratory” Ames Iowa (July 2004)

· Conference Panelist: Women in Science and Engineering, University of Washington (2003, 2004)

· Panelist: “Preparing Chemical Leaders of Tomorrow: Re-envisioning the Ph.D. University of Washington; Funded by the Dreyfus Foundation (March 2003)

· Conference Presentation: 12^th Annual Women in Science and Engineering Conference “The Path to a Faculty Career” University of Washington (Jan 2003)

· Conference Participant: AACC Oak Ridge Conference “The post Genome Era: New Targets and New Technologies” Seattle WA (May 2001)

· Conference Presentation/Session Presider: Northwest Regional ACS Meeting, Seattle WA (June 2001)

· Conference Co-author: “ Preparing for the professoriate: A graduate student’s perspective” ACS National meeting San Francisco with K.E. Miller (June 2000)

· Conference Presentation: University of Texas Teleconference Network, broadcast nationally “Fetal Hemolytic Disease” (September 1999)

· Conference Presentation: Washington College Chemistry Teachers Association “ Promoting an Understanding of Pressure in General Chemistry” with Vicky Minderhout, Ellensburg WA (April 1999)

· Conference Presentation: University of Texas Teleconference Network, broadcast nationally “A Primer on Paternity Testing ” (September 1998)

· Member of Project Kaleidoscope; Faculty for the 21^st Century (1998- present)

· Expert Witness related to medical/legal issues in Superior/Tribal/Military court jurisdictions, case names provided upon request (1991-present)

· Qualified Inspector for College of American Pathologists: Chemistry and Molecular Biology, various inspection teams (1990-2003)

· Invited Reviewer for Peer Reviewed Journals Analytical Chemistry, Talanta, Clinical Chemistry, Journal of Chemical Education, averaging 4 manuscripts/year (1990-present)

· Conference Presentation at Northwest Medical Laboratory Symposium American Society of Clinical Laboratory Scientists (ASCLS) “Laboratory Insiders View of Paternity Testing” (Nov 1998)

· Conference Presentation: ASCLS National Meeting “DNA testing in a clinical laboratory: Using PCR for the diagnosis of genetic diseases such as Rh Incompatibility” Atlanta GA (July 1997)

· Pacific Northwest Genetics Groups (PACNORGG), Member DNA Committee (1995-2001)

· Participating Presenter, Expanding Your Horizons, Bellevue Community College (1995-97)

· Clinical Laboratory Educators Teleconference, Secretary, (1996)

Internal to Seattle University

· School Personnel Committee, College of Science and Engineering (2003-2006)

· Member of Seattle University Science and Engineering Facilities Task Force (2006)

· Bannan Scholars Program Invited Lecturer, “Proteomics and Analytical Chemistry” (Oct 2005)

· Presentation: Overview of Chemistry Facilities to Seattle University 1) Dean’s Council 2) Executive Team and 3) Academic Assembly (2005-2006)

· Chemistry Department Search Committees (2000, 2001, 2003, 2004, 2005)

· Faculty Participant Seattle University School of Law Forensic Expert Class, Dr. John Mitchell and Dr. John Straight Coordinating Faculty (2001-2006)

· Panelist Seattle University New Faculty Institute “Making Scholarship a Priority” (2004, 2005)

· Seattle University Criminal Justice Search Committee (2004-2005)

· Seattle University Core Revitalization Subcommittee, Science and Engineering (2001)

· Seattle University Dean’s Search Advisory Committee Chair (1998)

· Pauling Award Committee, ACS, Seattle University (1996)

EXPERIENCE AND EXPERTISE

· Chemical methods of analysis including atomic spectroscopy, high performance liquid chromatography, mass spectrometry, electrophoresis and immunoassay. Development and validation of spectroscopic and chromatographic methods.

· Teaching general, analytical chemistry and organic chemistry laboratory and introductory laboratory medicine, forensic science and medical technology. Extensive interests in collaborative learning and community outreach in the area of science education.

· Laboratory techniques for Southern blot and Polymerase Chain Reaction (PCR) based methods of DNA analysis. Protein analysis using 2-D capillary electrophoresis of single cells

· Interpretation of DNA data for the diagnosis of:

· Alpha thalassemia

· Cystic fibrosis

· Huntington’s disease

· Sickle Cell disease

· Fragile X syndrome

· Maternal cell contamination

· Prader Willi syndrome

· Fetal Rh Typing

· Chronic Myelogenous Leukemia

· Ewing’s Sarcoma

· Cat Scratch Disease

· Rhabdomysosarcoma

· Twin zygosity

· Viral load testing for HIV/ HCV

· Uniparental disomy studies

· DNA identity determinations

Research Support; GRANTS AND AWARDS

· Sherman Fairchild Foundation co-PI “Promoting the development of problem solving skills in undergraduate science students at Seattle University” Awarded June 2006 (Award Amount $500,000 divided between Chemistry/Physics/Biology).

· Murdock Foundation author on behalf of the institution writing portions of two funded proposals “Faculty Startup for Analytical Chemistry ($25,000, funded 2006) and “Ramp-Up Grants ( $145,000, funded 2004).

· Merck Foundation/AAAS: “Promoting the Synergy between Chemistry and Biology at Seattle University” Awarded February 2005 (Award Amount: $60,000 (three years, plus institutional match)

· Seattle University Provost Assessment Award: Benchmarking the Seattle University Chemistry department by Comparison with a Variety of Undergraduate Institutions” Awarded 2004 (Award Amount $5,000)

· Bannan Foundation Equipment Award: “Atomic Absorption Spectrophotometry” Awarded 2004 (Award Amount $16,000)

· Core Revitilizion Award “An Interdisciplinary Core Course Based on Forensic Science” : CO-PI with Dr. Jackie Helfgott Awarded June 2001 (Award Amount $5000)

· Research Corporation Grant: “Modeling Surfactant Protein Interactions with Dynamic Surface Tension Analysis” Awarded November 2000 (Award Amount: $27,000 plus $17,000 institutional match)

· Dreyfus Foundation Grant: “Using the World of the Working Chemist to Reform the Analytical Chemistry Laboratory” Awarded January 2000 (Award Amount $20,000 plus $11,000 institutional)

· Huckabay Foundation “Preparing Future Faculty” Fellowship awarded to Keith Miller, mentored Dr. Miller while he was a Huckabay Fellow at Seattle University, Fall 1999

· Bannan Foundation Equipment Award, “Gas Chromatographic Equipment for Chemical Separations and Detection in the Chemistry and Environmental Engineering Departments at Seattle University” Awarded 1998 (Award Amount $12,000)

· Bannan Foundation Equipment Award, “Fluorescence and Fluorescence Polarization Equipment for Biochemical Analysis at Seattle University” 1999 (Award Amount $21,000)

· Bannan Foundation Equipment Award, “Chromatographic Equipment for Surface Tension Analysis in the Chemistry at Seattle University” 1999 (Award Amount $9,500)

· Van Slyke Society American Association of Clinical Chemistry Research Grant, “ Rapid Examination and Determination of Surfactants by Simultaneous Surface Tension and Surface Adsorption Analysis”, Awarded 1996-7 (Award amount $10,000)

· Summer Faculty Fellowship, 1997, 2001; Faculty Development Award, Seattle University, 1997, 1998

· Young Investigator Award, ACLPS (Academy of Clinical Laboratory Physicians and Scientists), 1989

· Pace Academic Graduate Scholarship, Iowa State University, 1982-1985,

· Outstanding Teaching Award, Colorado State University, 1982

· Scholarship recipient: President’s scholar and Lelia Morgan Women’s Scholarship, CSU 1978-82

· Elected to SPURS, Mortar Board, and Phi Kappa Phi, 1982

SELECTED EDUCATIONAL ACTIVITIES DATES

Courses Taught at Seattle University

Quantitative Analysis Lecture and Laboratory 1995-2006 biyearly

Instrumental Analysis Lecture and Laboratory C326 1995-2004 annually

General Chemistry Lecture for Majors (C121 series) 1997-1999 biyearly

General Chemistry Laboratory C133 1997-2000 yearly

Organic Chemistry Laboratories 1995-2000 yearly

Forensic Evidence (contributing faculty) 1999-2006 yearly

Forensic Science 2002-2004 + contributing faculty

Average Student Evaluation of Teaching from relevant teaching performance questions= 4.3 on a 5.0 scale

B. Peer-reviewed publications

1. Bramanti, E; Allegrini, C.; Onor, M; Raspi, G; Skogerboe, KJ; Synovec, RE; Flow Injection Analysis with Diode Array Absorbance Detection and Dynamic Surface Tension Detection for Studying Denaturation and Surface Activity of Globular Proteins Analytical Biochemistry, 2006, 351: 100-113.

2. Bramanti, E; Allegrini, C.; Skogerboe, KJ; Synovec, RE; High-Throughput Screening of Protein Surface Activity via Flow Injection Analysis-pH Gradient-Dynamic Surface Tension Detection Anal. Chem., 2005; 77(1); 250-258.

3. Souter V,Kapur RP, Nyholt DR, Skogerboe KJ, Myerson D,Ton CC,Opheim KE,Easterling TR, Shields LE, Montgomery,GW, Glass IA. A Report of Dizygous Monochorionic Twins N Engl J Med 2003;349:154-8.

4. Miller KE, Bramanti. Prazen BJ, Prezhdo, M, .Skogerboe, KJ, Synovec RE “Multidimensional Analytical of Polyethylene Glycols by Size Exclusion Chromatography and Dynamic Surface Tension Detection” Anal. Chem., 2000, 72, 4372-4380.

5. Miller KE, Skogerboe, KJ, Synovec RE “Novel Calibration of a Dynamic Surface Tension Detector: Flow Injection Analysis of Kinetically-Hindered Surface Active Analytes”, Talanta, 50:1045-56, 1999.

6. Olson, NA, Skogerboe, KJ, Synovec RE “ Hydrophobic Interaction Chromatography Coupled with Dynamic Surface Tension Detection for the Determination of Surface Active Species in Protein Formulations” Journal of Chromatography A, 806: 239-50, 1998.

7. Olson, NA, Synovec RE, Bond WB, Alloway DM, Skogerboe KJ “Dynamic Surface Tension and Surface Adhesion Detection for the Rapid Analysis of Surfactants in Flowing Aqueous Liquids“ Anal. Chem. 69: 3496-3505, 1997.

8. Anderson DA, Vanlente AA, Skogerboe KJ et al. “Fundamental Reviews in Clinical Chemistry” Anal Chem 65: R416-419, 1997.

9. Keitges EA, Skogerboe KJ, Luthardt FW, “Mosaic trisomy 14 diagnosed at amniocentesis, confirmed in CVS cultures, but absent in fetal skin cultures”Cytogent Cell Genet 63, 248, 1994.

10. Conway KA, Sako J, Skogerboe KJ. “Preparation of Genomic DNA suitable for Restriction Enzyme Digestion from Whole Blood Collected with Heparin as an Anticoagulant” Clin.Chem. 42, 1995.

11. Anderson DA, Vanlente AA, Skogerboe KJ et al. “Fundamental Reviews in Clinical Chemistry” Anal Chem 65: R416-419, 1993.

12. Skogerboe KJ, West SF, Smith C, LeCrone C, Terashita S, Detter JD, Tait JF. “BCB inclusion bodies: In Reply,” Arch Path Lab Med 117, 1993.

13. Skogerboe KJ, West SF, Smith C, LeCrone C, Terashita S, Detter JD, Tait JF. “BCB inclusion bodies: relation to DNA-determination genotype,” Arch Path Lab Med 116:1012-8, 1992.

14. West SF, Skogerboe KJ, LeCrone C, Detter JD. “Prenatal diagnosis of Hb Bart’s Hydrops Fetalis”, Clinical Laboratory Science 5:53-6, 1992.

15. Skogerboe KJ, West SF, Murillo MD, Glass MW, Shaunak S, Tait JF. “Newborn genetic screening for sickle cell disease: correlation of DNA analysis with hemoglobin electrophoresis,” Clin Chem 37:454-8, 1991.

16. Skogerboe KJ, West SF, Murillo MD, Tait JF. “Development and evaluation of a simplified method for detection of the delta F508 mutation in cystic fibrosis,” Clin Chem 36:1984-6, 1990.

17. Skogerboe KJ, Felix-Slinn T, Synovec RE. “Ion chromatographic determination of oxalate in Plasma: Correlation study with an enzymatic method,” Anal Chim Acta 237:299-304, 1990.

18. Skogerboe KJ, West SF, Murillo MD, Tait JF. “PCR dot blots: Large signal differences between sense and anti-sense probes,” BioTechniques 9:154-6, 1990.

19. Skogerboe KJ, Labbe RF, Rettmer RL, Sundquist JP, Gargett AM. “Chemiluminescent measurement of total urinary nitrogen for accurate calculation of nitrogen balance,” Clin Chem 36:752-5, 1990.

20. Howard JD, Skogerboe KJ, Case GA, Raisys VA, Lacsina EQ. “Death following accidental sodium azide ingestion,” J Forensic Toxicology 35:193-6, 1990.

21. Skogerboe KJ, McLaughlin MK, Labbe RF. “Comparison of sample preparation techniques for the measurement of total nitrogen in stool and food samples,” Clin Chem 35:1114, 1989.

22. Skogerboe KJ, Johnson C, Labbe RF. “Resolution of several issues in the HPLC analysis of urinary porphyrins,” Clin Chem 34:1115, 1989.

23. Labbe, RF, Skogerboe KJ, Rettmer RL, Davis HA. “Laser photobioactivation mechanisms: In-vitro studies using ascorbic acid uptake and hydroxyproline formation as biochemical markers of irradiation response,” J Lasers Surg Med 10:201-7, 1990.

24. Skogerboe KJ. “Contributions of Analytical Chemistry to the Clinical Laboratory,” Anal Chem 60:1271A-5A, 1988.

25. Skogerboe KJ, Raisys VA, Friel PN, Yerby MS. “A method for in-vitro prediction of phenytoin toxicity,” Clin Chem 33:1019, 1987.

26. Skogerboe KJ, Yeung ES. “Straylight reduction in fiber optic probes,” Anal Chem 59:1812-4, 1987.

27. Skogerboe KJ, Yeung ES. “Single laser thermal lens detector for microbore liquid chromatography based on high frequency modulation,” Anal Chem 58:1014-8, 1986.

28. Skogerboe KJ, Yeung ES. “Quantitative gas chromatography without analyte identification by ultrasonic detection,” Anal Chem 56:2684-6, 1984.

29. Miller DA, Skogerboe KJ, Grimsrud EP. “Enhancement of electron capture detector response to polycyclic aromatic and related hydrocarbons by addition of oxygen to carrier gas,” <